100 Section - Functionally improved extracellular vesicles enhance the bone regeneration for glucocorticoid-induced osteonecrosis of the femoral head via miR-30d-5p/Cyp24a1 axis

Tuesday, May 19, 2026

6:00 PM - 7:30 PM PT

Presenting Author/Autor expositor(s)

XX

Xiao-na Xiang, PhD

Physical therapist
West China Hospital of Sichuan University
Chengdu, Sichuan, China (People's Republic)

Co-Author/Coautor(s)

JZ

Jiang-Yin Zhang, MS

Physical therapist
West China Hospital of Sichuan University
Chengdu, Sichuan, China (People's Republic)

Corresponding Author/Autor a quien dirigir la corr(s)

CH

Chengqi He, MD

professor
Sichuan University West China Hospital Rehabilitation Medical Center
Chengdu, Sichuan, China (People's Republic)

Objectives : Regeneration of femoral head structure in osteonecrosis of the femoral head (ONFH) are thorny problems. Extracellular vesicles (EVs) and pulsed electromagnetic fields (PEMFs) precondition method may be helpful to promote its regeneration. Hence, we reported the engineered extracellular vesicles derived from BMSCs under pulsed electromagnetic fields (BMSC-EVs^PEMFs) in treating ONFH.

Design: To determine the effect and underlying mechanisms of BMSC-EVs^PEMFs in treating ONFH, we conducted serial research. We used PEMFs with amplitudes of a 1, 1,6, and 3 millitesla (mT) for designing different BMSC-EVs^PEMFs and co-cultured them with pre-osteoblasts. Then, we screened the proper BMSC-EVs^PEMFs by assessing its effect on promoting proliferation and osteogenesis. Then, we conduced animal study for a further confirmation, enabling us to analyze both effectiveness on ONFH and biocompatibility. Furthermore, we conducted miRNA transcriptome sequencing and dual-luciferase reporter assay to investigate a key miRNA and targeted gene. To explore the role of this key miRNA in treating ONFH, we used miRNA mimic, inhibitor, and targeted gene siRNA in cell experiment and used agomir in animal experiment.

Results: Our findings revealed that BMSC-EVs^PEMFs with 3 mT enhanced the proliferation and osteogenesis of pre-osteoblasts stimulated by glucocorticoid and recovered the bone structure of femoral head in ONFH rats. The results of miRNA sequencing showed that miR-30d-5p was increased in BMSC-EVs^PEMFs with 3 mT, which inhibited the expression of CYP24A1. Extra addition of miR-30d-5p enhanced the osteogenesis of pre-osteoblasts in vitro and promoted bone regeneration and angiogenesis of femoral head in ONFH model.

Conclusion: BMSC-EVs^PEMFs with 3 mT promoted osteogenesis of pre-osteoblasts via miR-30d-5p/CYP24A1 axis in ONFH model. PEMFs, as a pre-condition strategy, could change the EVs cargo, and adding miR-30d-5p may be a promising complementary strategy against ONFH.